human bax gene Search Results


bax  (Sino Biological)
92
Sino Biological bax
a, Schematic for the surface immunoprecipitation (IP) of BCL2 family proteins. Anti-apoptotic proteins (BCL2, <t>BCLxL,</t> <t>MCL1)</t> in cell extracts were lysed with mild detergent and immobilized onto the surface of the reaction chamber. b, Schematic of sandwich-type immunoassay selectively detecting PPI complex (left) and the total level of surface bait (right). The fluorescence signals from detection antibodies were imaged by a single-molecule co-IP platform. c, Comparing the formation of BCL2-BIM BH3 PPI complex in an intracellular environment by BCL2-mCherry/BIM BH3 -eGFP co-transfection (red) and in vitro mixing of two individual extracts expressing BCL2-mCherry and BIM BH3 -eGFP (black). d, Relative changes of active <t>BAX</t> level from the STS treated HL60 cells after lysed with different detergent types. The monoclonal antibody 6A7 was used to detect the activated BAX . Spurious BAX activation by Triton X-100 was indicated (blue). e, Surface-immobilization of the matching IP antibodies immunoprecipitate BCL2-eGFP from the crude extract with high specificity. f, Comparison of signal to noise ratio (SNR) from single molecule counting analysis (red) and total fluorescence signal integrating analysis (black). The raw data to calculate the SNRs are described in Extended Data Fig. 2. g, Counts of endogenous BCL2-related PPI complexes (BCL2-BIM, BCL2-BAX) from the 30,000 HL60 cells by using immunoassay. Error bars represent mean ± s.d. from independent inter-chip measurement ( n ≥ 2). (Two-sided two-sample t -test, P =1.46x10 - , 1.11x10 - respectively). h , Counts of endogenous BCL2-BIM PPI complexes and inter-chip CVs from the fixed numbers of HL60 cells ( n ≥ 2). i , Inter-chip CVs obtained from independent inter-chip measurement for all the immunoassays and cell numbers ( n ≥ 2).
Bax, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bax/product/Sino Biological
Average 92 stars, based on 1 article reviews
bax - by Bioz Stars, 2026-05
92/100 stars
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human bax gene  (Incyte corporation)
90
Incyte corporation human bax gene
a, Schematic for the surface immunoprecipitation (IP) of BCL2 family proteins. Anti-apoptotic proteins (BCL2, <t>BCLxL,</t> <t>MCL1)</t> in cell extracts were lysed with mild detergent and immobilized onto the surface of the reaction chamber. b, Schematic of sandwich-type immunoassay selectively detecting PPI complex (left) and the total level of surface bait (right). The fluorescence signals from detection antibodies were imaged by a single-molecule co-IP platform. c, Comparing the formation of BCL2-BIM BH3 PPI complex in an intracellular environment by BCL2-mCherry/BIM BH3 -eGFP co-transfection (red) and in vitro mixing of two individual extracts expressing BCL2-mCherry and BIM BH3 -eGFP (black). d, Relative changes of active <t>BAX</t> level from the STS treated HL60 cells after lysed with different detergent types. The monoclonal antibody 6A7 was used to detect the activated BAX . Spurious BAX activation by Triton X-100 was indicated (blue). e, Surface-immobilization of the matching IP antibodies immunoprecipitate BCL2-eGFP from the crude extract with high specificity. f, Comparison of signal to noise ratio (SNR) from single molecule counting analysis (red) and total fluorescence signal integrating analysis (black). The raw data to calculate the SNRs are described in Extended Data Fig. 2. g, Counts of endogenous BCL2-related PPI complexes (BCL2-BIM, BCL2-BAX) from the 30,000 HL60 cells by using immunoassay. Error bars represent mean ± s.d. from independent inter-chip measurement ( n ≥ 2). (Two-sided two-sample t -test, P =1.46x10 - , 1.11x10 - respectively). h , Counts of endogenous BCL2-BIM PPI complexes and inter-chip CVs from the fixed numbers of HL60 cells ( n ≥ 2). i , Inter-chip CVs obtained from independent inter-chip measurement for all the immunoassays and cell numbers ( n ≥ 2).
Human Bax Gene, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human bax gene/product/Incyte corporation
Average 90 stars, based on 1 article reviews
human bax gene - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
human bax gene orf cdna clone in cloning vector  (Sino Biological)
92
Sino Biological human bax gene orf cdna clone in cloning vector
a, Schematic for the surface immunoprecipitation (IP) of BCL2 family proteins. Anti-apoptotic proteins (BCL2, <t>BCLxL,</t> <t>MCL1)</t> in cell extracts were lysed with mild detergent and immobilized onto the surface of the reaction chamber. b, Schematic of sandwich-type immunoassay selectively detecting PPI complex (left) and the total level of surface bait (right). The fluorescence signals from detection antibodies were imaged by a single-molecule co-IP platform. c, Comparing the formation of BCL2-BIM BH3 PPI complex in an intracellular environment by BCL2-mCherry/BIM BH3 -eGFP co-transfection (red) and in vitro mixing of two individual extracts expressing BCL2-mCherry and BIM BH3 -eGFP (black). d, Relative changes of active <t>BAX</t> level from the STS treated HL60 cells after lysed with different detergent types. The monoclonal antibody 6A7 was used to detect the activated BAX . Spurious BAX activation by Triton X-100 was indicated (blue). e, Surface-immobilization of the matching IP antibodies immunoprecipitate BCL2-eGFP from the crude extract with high specificity. f, Comparison of signal to noise ratio (SNR) from single molecule counting analysis (red) and total fluorescence signal integrating analysis (black). The raw data to calculate the SNRs are described in Extended Data Fig. 2. g, Counts of endogenous BCL2-related PPI complexes (BCL2-BIM, BCL2-BAX) from the 30,000 HL60 cells by using immunoassay. Error bars represent mean ± s.d. from independent inter-chip measurement ( n ≥ 2). (Two-sided two-sample t -test, P =1.46x10 - , 1.11x10 - respectively). h , Counts of endogenous BCL2-BIM PPI complexes and inter-chip CVs from the fixed numbers of HL60 cells ( n ≥ 2). i , Inter-chip CVs obtained from independent inter-chip measurement for all the immunoassays and cell numbers ( n ≥ 2).
Human Bax Gene Orf Cdna Clone In Cloning Vector, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human bax gene orf cdna clone in cloning vector/product/Sino Biological
Average 92 stars, based on 1 article reviews
human bax gene orf cdna clone in cloning vector - by Bioz Stars, 2026-05
92/100 stars
  Buy from Supplier
human bax gene orf cdna clone expression plasmid c flag tag  (Sino Biological)
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Full length Clone DNA of Human BCL2 associated X protein with C terminal Flag tag
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human bax gene orf cdna clone expression plasmid c ha tag  (Sino Biological)
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Full length Clone DNA of Human BCL2 associated X protein with C terminal HA tag
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human bax gene orf cdna clone expression plasmid c gfpspark tag  (Sino Biological)
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Full length Clone DNA of Human BCL2 associated X protein with C terminal GFPSpark tag
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human bax gene orf cdna clone expression plasmid c myc tag  (Sino Biological)
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Full length Clone DNA of Human BCL2 associated X protein with C terminal Myc tag
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human bax gene orf cdna clone expression plasmid  (Sino Biological)
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Full length Clone DNA of Human BCL2 associated X protein
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bax human gene knockout kit  (OriGene)
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BAX KN2 0 Human gene knockout kit via CRISPR non homology mediated
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human bax gene orf cdna clone expression plasmid n his tag  (Sino Biological)
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Full length Clone DNA of Human BCL2 associated X protein with N terminal His tag
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human bax gene orf cdna clone expression plasmid c his tag  (Sino Biological)
N/A
Full length Clone DNA of Human BCL2 associated X protein with C terminal His tag
   Buy from Supplier
human bax gene orf cdna clone expression plasmid n gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human BCL2 associated X protein with N terminal GFPSpark tag
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Image Search Results


a, Schematic for the surface immunoprecipitation (IP) of BCL2 family proteins. Anti-apoptotic proteins (BCL2, BCLxL, MCL1) in cell extracts were lysed with mild detergent and immobilized onto the surface of the reaction chamber. b, Schematic of sandwich-type immunoassay selectively detecting PPI complex (left) and the total level of surface bait (right). The fluorescence signals from detection antibodies were imaged by a single-molecule co-IP platform. c, Comparing the formation of BCL2-BIM BH3 PPI complex in an intracellular environment by BCL2-mCherry/BIM BH3 -eGFP co-transfection (red) and in vitro mixing of two individual extracts expressing BCL2-mCherry and BIM BH3 -eGFP (black). d, Relative changes of active BAX level from the STS treated HL60 cells after lysed with different detergent types. The monoclonal antibody 6A7 was used to detect the activated BAX . Spurious BAX activation by Triton X-100 was indicated (blue). e, Surface-immobilization of the matching IP antibodies immunoprecipitate BCL2-eGFP from the crude extract with high specificity. f, Comparison of signal to noise ratio (SNR) from single molecule counting analysis (red) and total fluorescence signal integrating analysis (black). The raw data to calculate the SNRs are described in Extended Data Fig. 2. g, Counts of endogenous BCL2-related PPI complexes (BCL2-BIM, BCL2-BAX) from the 30,000 HL60 cells by using immunoassay. Error bars represent mean ± s.d. from independent inter-chip measurement ( n ≥ 2). (Two-sided two-sample t -test, P =1.46x10 - , 1.11x10 - respectively). h , Counts of endogenous BCL2-BIM PPI complexes and inter-chip CVs from the fixed numbers of HL60 cells ( n ≥ 2). i , Inter-chip CVs obtained from independent inter-chip measurement for all the immunoassays and cell numbers ( n ≥ 2).

Journal: bioRxiv

Article Title: Predicting the efficacy of BH3 mimetics through the profiling of multiple protein complexes

doi: 10.1101/2023.01.16.524337

Figure Lengend Snippet: a, Schematic for the surface immunoprecipitation (IP) of BCL2 family proteins. Anti-apoptotic proteins (BCL2, BCLxL, MCL1) in cell extracts were lysed with mild detergent and immobilized onto the surface of the reaction chamber. b, Schematic of sandwich-type immunoassay selectively detecting PPI complex (left) and the total level of surface bait (right). The fluorescence signals from detection antibodies were imaged by a single-molecule co-IP platform. c, Comparing the formation of BCL2-BIM BH3 PPI complex in an intracellular environment by BCL2-mCherry/BIM BH3 -eGFP co-transfection (red) and in vitro mixing of two individual extracts expressing BCL2-mCherry and BIM BH3 -eGFP (black). d, Relative changes of active BAX level from the STS treated HL60 cells after lysed with different detergent types. The monoclonal antibody 6A7 was used to detect the activated BAX . Spurious BAX activation by Triton X-100 was indicated (blue). e, Surface-immobilization of the matching IP antibodies immunoprecipitate BCL2-eGFP from the crude extract with high specificity. f, Comparison of signal to noise ratio (SNR) from single molecule counting analysis (red) and total fluorescence signal integrating analysis (black). The raw data to calculate the SNRs are described in Extended Data Fig. 2. g, Counts of endogenous BCL2-related PPI complexes (BCL2-BIM, BCL2-BAX) from the 30,000 HL60 cells by using immunoassay. Error bars represent mean ± s.d. from independent inter-chip measurement ( n ≥ 2). (Two-sided two-sample t -test, P =1.46x10 - , 1.11x10 - respectively). h , Counts of endogenous BCL2-BIM PPI complexes and inter-chip CVs from the fixed numbers of HL60 cells ( n ≥ 2). i , Inter-chip CVs obtained from independent inter-chip measurement for all the immunoassays and cell numbers ( n ≥ 2).

Article Snippet: All BCL2 family proteins ( BCL2 (HG10195-M; SinoBiological), BCLxL (HG10455-M; SinoBiological), MCL1 (HG10240-M; SinoBiological), BIM EL (HG13816-G; SinoBiological), BAD (HG10020-M; SinoBiological), BAX (HG11619-M; SinoBiological), BAK (HG10450-M; SinoBiological) and NOXA (HG16548-U; SinoBiological)) were isolated from their respective human cDNA.

Techniques: Immunoprecipitation, Fluorescence, Co-Immunoprecipitation Assay, Cotransfection, In Vitro, Expressing, Activation Assay, Single Molecule Counting

Changes of endogenous PPI profiles of HL60 cells through the apoptosis progression by 2 μM of STS. (a) Active BAX/BAK level, (b) Total levels of anti-apoptotic proteins (BCL2, BCLxL, MCL1), (c) BIM complexes with anti-apoptotic proteins, (d) BAX complexes with anti-apoptotic proteins. e , Schematic for the PPI probe binding assay (PBA) for measuring unoccupied populations of surface-immobilized anti-apoptotic proteins. f, Changes of BIM BH3 PBAs for anti- apoptotic proteins from HL60 cells through the apoptosis progression by STS. g, Schematic for the comparison of the BCL2 PPI complex compositions in different AML cell lines. The density of surface-immobilized BCL2 was constantly maintained by optimizing the total protein concentration of crude cell extracts for all AML cell lines (HL60, THP-1, NB4, U937). h, Compositions of the BCL2 complexes (BIM, BAX, BAD, BAK) in four different AML cell lines. The labeling efficiencies of the immunoassays were applied. i, BCL2-BIM BH3 PBA counts of four AML cell lines. j, The PPI complex compositions of BCL2 in HL60, NB4, and U937 cell extracts. k, Schematic of binding competition between two PPI probes, BIM BH3 -eGFP and BAD- mCherry, on surface immobilized BCL2 from HL60 cells. l, Changes of BCL2-BIM BH3 PBA and BCL2-BAD PBA with increasing amounts of BAD probe. BIM BH3 PPI probe was presented in 10 nM.

Journal: bioRxiv

Article Title: Predicting the efficacy of BH3 mimetics through the profiling of multiple protein complexes

doi: 10.1101/2023.01.16.524337

Figure Lengend Snippet: Changes of endogenous PPI profiles of HL60 cells through the apoptosis progression by 2 μM of STS. (a) Active BAX/BAK level, (b) Total levels of anti-apoptotic proteins (BCL2, BCLxL, MCL1), (c) BIM complexes with anti-apoptotic proteins, (d) BAX complexes with anti-apoptotic proteins. e , Schematic for the PPI probe binding assay (PBA) for measuring unoccupied populations of surface-immobilized anti-apoptotic proteins. f, Changes of BIM BH3 PBAs for anti- apoptotic proteins from HL60 cells through the apoptosis progression by STS. g, Schematic for the comparison of the BCL2 PPI complex compositions in different AML cell lines. The density of surface-immobilized BCL2 was constantly maintained by optimizing the total protein concentration of crude cell extracts for all AML cell lines (HL60, THP-1, NB4, U937). h, Compositions of the BCL2 complexes (BIM, BAX, BAD, BAK) in four different AML cell lines. The labeling efficiencies of the immunoassays were applied. i, BCL2-BIM BH3 PBA counts of four AML cell lines. j, The PPI complex compositions of BCL2 in HL60, NB4, and U937 cell extracts. k, Schematic of binding competition between two PPI probes, BIM BH3 -eGFP and BAD- mCherry, on surface immobilized BCL2 from HL60 cells. l, Changes of BCL2-BIM BH3 PBA and BCL2-BAD PBA with increasing amounts of BAD probe. BIM BH3 PPI probe was presented in 10 nM.

Article Snippet: All BCL2 family proteins ( BCL2 (HG10195-M; SinoBiological), BCLxL (HG10455-M; SinoBiological), MCL1 (HG10240-M; SinoBiological), BIM EL (HG13816-G; SinoBiological), BAD (HG10020-M; SinoBiological), BAX (HG11619-M; SinoBiological), BAK (HG10450-M; SinoBiological) and NOXA (HG16548-U; SinoBiological)) were isolated from their respective human cDNA.

Techniques: Binding Assay, Protein Concentration, Labeling

Changes of BCL2-related PPI profile in HL60 cells after ABT-199 (100 nM, 24 hours) treatment. (a) Total levels of anti-apoptotic proteins, (b) Active BAX/BAK level, (c) Total levels of BAX/BAK, (d) BCL2 complexes (BIM, BAX), (e) BCL2-BIM BH3 PBA. f-h, Changes of PPI profile of HL60 cells after higher concentration of ABT-199 (300 nM) treatment. (f) BCL2 complexes (BIM, BAX), (g) Active BAX/BAK level, (h) Total levels of BAX/BAK. i, Schematic model of mode of action of ABT-199. j-l , Changes of MCL1-related PPI profile of U937 cells after AZD-5991 (100 nM, 24 hours) treatment. (j) Active BAX/BAK level, (k) MCL1 complexes (BIM, BAK), (l) MCL1-BIM BH3 PBA. The decrease of MCL1-BAK complex after AZD-5991 treatment was indicated (blue). m, Changes of BCLxL complexes (BIM, BAX, BAD, BAK) from U937 cells after AZD-5991 (100 nM, 24 hours) treatment. The increase of BCLxL-BAK complex after AZD-5991 treatment was indicated (green). n, Comparison of relative changes of BAK complexes from U937 cells after AZD-5991 treatment. The relative changes were obtained from the indicated data in (k) and (m). o, Schematic model of mode of action of AZD-5991. (d, k, m) The labeling efficiencies of the immunoassays were applied.

Journal: bioRxiv

Article Title: Predicting the efficacy of BH3 mimetics through the profiling of multiple protein complexes

doi: 10.1101/2023.01.16.524337

Figure Lengend Snippet: Changes of BCL2-related PPI profile in HL60 cells after ABT-199 (100 nM, 24 hours) treatment. (a) Total levels of anti-apoptotic proteins, (b) Active BAX/BAK level, (c) Total levels of BAX/BAK, (d) BCL2 complexes (BIM, BAX), (e) BCL2-BIM BH3 PBA. f-h, Changes of PPI profile of HL60 cells after higher concentration of ABT-199 (300 nM) treatment. (f) BCL2 complexes (BIM, BAX), (g) Active BAX/BAK level, (h) Total levels of BAX/BAK. i, Schematic model of mode of action of ABT-199. j-l , Changes of MCL1-related PPI profile of U937 cells after AZD-5991 (100 nM, 24 hours) treatment. (j) Active BAX/BAK level, (k) MCL1 complexes (BIM, BAK), (l) MCL1-BIM BH3 PBA. The decrease of MCL1-BAK complex after AZD-5991 treatment was indicated (blue). m, Changes of BCLxL complexes (BIM, BAX, BAD, BAK) from U937 cells after AZD-5991 (100 nM, 24 hours) treatment. The increase of BCLxL-BAK complex after AZD-5991 treatment was indicated (green). n, Comparison of relative changes of BAK complexes from U937 cells after AZD-5991 treatment. The relative changes were obtained from the indicated data in (k) and (m). o, Schematic model of mode of action of AZD-5991. (d, k, m) The labeling efficiencies of the immunoassays were applied.

Article Snippet: All BCL2 family proteins ( BCL2 (HG10195-M; SinoBiological), BCLxL (HG10455-M; SinoBiological), MCL1 (HG10240-M; SinoBiological), BIM EL (HG13816-G; SinoBiological), BAD (HG10020-M; SinoBiological), BAX (HG11619-M; SinoBiological), BAK (HG10450-M; SinoBiological) and NOXA (HG16548-U; SinoBiological)) were isolated from their respective human cDNA.

Techniques: Concentration Assay, Labeling

a, Schematic for generating linear regression correlation between ex vivo efficacy of ABT-199 and PPI profiles from primary AML samples. The collected AML cells were cultured and treated with 0-1 μM of ABT-199, and the area under curves (AUCs) of cell viability were obtained as ex vivo drug efficacy (upper). ∼1.2x10 of primary AML cells on the same cohort were performed single-molecule co-IP profiling (lower) ( n =32). b , Pearson’s correlations between ex vivo AUC of ABT-199 and PPI profiles for primary AML biopsies. c, d , Correlations between ex vivo AUC and single BCL2-related PPI metrics. (c) BCL2-BAX complex, (d) BCL2- BIM complex. e, Correlation between ex vivo AUC and combination of multiple PPI metrics (BCL2-BIM BH3 PBA, BCL2-BAX CPX, and BCLxL-BAK CPX). The statistical indicators (coefficient, p -value) of each metric were displayed. f, Clinical features and the ABT-199 administration history of BC-7064. g, Comparison of the PPI profiles from the initial and the relapsed BC-7064 samples. h, The estimated scores of BC-7064 and BC-7064-R from model (e). i, Investigating the change of the estimated score and ex vivo AUC depending on the relapse of the BC-7064.

Journal: bioRxiv

Article Title: Predicting the efficacy of BH3 mimetics through the profiling of multiple protein complexes

doi: 10.1101/2023.01.16.524337

Figure Lengend Snippet: a, Schematic for generating linear regression correlation between ex vivo efficacy of ABT-199 and PPI profiles from primary AML samples. The collected AML cells were cultured and treated with 0-1 μM of ABT-199, and the area under curves (AUCs) of cell viability were obtained as ex vivo drug efficacy (upper). ∼1.2x10 of primary AML cells on the same cohort were performed single-molecule co-IP profiling (lower) ( n =32). b , Pearson’s correlations between ex vivo AUC of ABT-199 and PPI profiles for primary AML biopsies. c, d , Correlations between ex vivo AUC and single BCL2-related PPI metrics. (c) BCL2-BAX complex, (d) BCL2- BIM complex. e, Correlation between ex vivo AUC and combination of multiple PPI metrics (BCL2-BIM BH3 PBA, BCL2-BAX CPX, and BCLxL-BAK CPX). The statistical indicators (coefficient, p -value) of each metric were displayed. f, Clinical features and the ABT-199 administration history of BC-7064. g, Comparison of the PPI profiles from the initial and the relapsed BC-7064 samples. h, The estimated scores of BC-7064 and BC-7064-R from model (e). i, Investigating the change of the estimated score and ex vivo AUC depending on the relapse of the BC-7064.

Article Snippet: All BCL2 family proteins ( BCL2 (HG10195-M; SinoBiological), BCLxL (HG10455-M; SinoBiological), MCL1 (HG10240-M; SinoBiological), BIM EL (HG13816-G; SinoBiological), BAD (HG10020-M; SinoBiological), BAX (HG11619-M; SinoBiological), BAK (HG10450-M; SinoBiological) and NOXA (HG16548-U; SinoBiological)) were isolated from their respective human cDNA.

Techniques: Ex Vivo, Cell Culture, Co-Immunoprecipitation Assay